Ultrastructure and viability of isolated bovine preantral follicles
نویسندگان
چکیده
منابع مشابه
Ultrastructure and viability of isolated bovine preantral follicles.
Techniques for the isolation of ovarian follicles and maturation of oocytes in vitro have enormous reproductive potential. Preservation of normal tissue function is vital. This study emphasizes the ultrastructure and viability of mechanically isolated bovine small (diameter 40-100 microm) preantral and large (140-450 microm) preantral/early antral follicles. Viability studies were performed for...
متن کاملIn vitro culture of bovine preantral follicles.
Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM) in tissue culture medium (TCM 199) suppleme...
متن کاملIn vitro culture of bovine preantral follicles: a review
Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular activation of small (primordial) preantral ...
متن کاملPhytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter ...
متن کاملP-36: The Effect of Lipoic Acid on Developmental Competence of Cultured Isolated Mouse Preantral Follicles
Background: α-Lipoic acid (LA) is a cofactor in mitochondrial multienzyme complexes and is well-known for its antioxidant properties in both of in vivo and in vitro condition. Materials and Methods: Preantral follicles were isolated from 2 weeks old NMRI mice ovaries. LA at concentrations of 0 and 100 μM was supplemented to culture media (α-MEM medium supplemented with 5% FBS, 100 mlU/ml FSH, 1...
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ژورنال
عنوان ژورنال: Human Reproduction Update
سال: 1998
ISSN: 1355-4786,1460-2369
DOI: 10.1093/humupd/4.6.833